Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it. Chloroquine production process What is plaquenil prescribed for How much does plaquenil cost Who can do plaquenil test Chloroquine is a lysosomotropic weak base, which in the monoprotonated form diffuses into the lysosome, where it becomes diprotonated and becomes trapped. Protonated chloroquine then changes the lysosomal pH, thereby inhibiting autophagic degradation in the lysosomes. GTX632501 ICC/IF Image LC3B antibody GT3612 detects LC3B protein at autophagosome by immunofluorescent analysis. Samples HeLa cells mock left and treated with 50μM Chloroquine for 24 hr right were fixed in 4% paraformaldehyde at RT for 15 min. Anti-LC3-I/II Antibody; Synonym Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha; find Sigma-Aldrich-ABC929 MSDS, related peer-reviewed papers, technical documents, similar products & more at. The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations. In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead. Lc3b chloroquine CST - Chloroquine, Anti-LC3B antibody GT3612 GTX632501 GeneTex Chloroquine cstPlaquenil erg LC3B Knockout Validation LC3B Antibody NB100-2220 - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line KO untreated - or treated + with 50 uM Chloroquine for 18 hours. The Role of LC3 within the Autophagic Pathway Antibody News.. Anti-LC3-I/II Antibody Sigma-Aldrich. How to Interpret LC3 Immunoblotting Autophagy Vol 3, No 6. Chloroquine is commonly used to study the role of endosomal acidification in cellular processes 2, 3, such as the signaling of intracellular TLRs. Moreover, Chloroquine inhibits autophagy as it raises the lysosomal pH, which leads to inhibition of both fusion of autophagosome with lysosome and lysosomal protein degradation. 1. LC3B-II Chloroquine LC3B-I HT-1080 A20 60 50 40 20 10 – – – +– HeLa mock +LC3B Western blot analysis of extracts from HeLa cells, mock transfected or transfected with rat LC3B, and from HT-1080 and A20 cells, untreated or chloroquine-treated 50 µM, overnight, using LC3B Antibody. Entrez-Gene ID # 81631 Swiss-Prot Acc. # Q9GZQ8 What is the difference between LC3A, LC3B and LC3C, or LC3-I and LC3-II? LC3 is a soluble protein with a molecular mass of ∼17 kDa and is distributed ubiquitously in eukaryotes. It is expressed as the splice variants LC3A, LC3B, and LC3C which display unique tissue distribution.