Confocal immunofluorescent analysis of He La (upper) and C2C12 (lower) cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (3 hr, middle) or untreated (right) using LC3A/B (D3U4C) XP Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using LC3A/B (D3U4C) XP® Rabbit m Ab in the presence of control peptide (left) or antigen-specific peptide (right). Western blot analysis of extracts from He La cells, mock transfected or transfected with rat LC3B, and from HT-1080 and A20 cells, untreated or chloroquine-treated (50 μM, overnight), using LC3B Antibody. Hydroxychloroquine buy online Hydroxychloroquine aplastic anemia Chloroquine is commonly used to study the role of endosomal acidification in cellular processes 2, 3, such as the signaling of intracellular TLRs. Moreover, Chloroquine inhibits autophagy as it raises the lysosomal pH, which leads to inhibition of both fusion of autophagosome with lysosome and lysosomal protein degradation 4. Chloroquine LC3B-I HT-1080 A20 60 50 40 20 10 – – – +– HeLa mock +LC3B Western blot analysis of extracts from HeLa cells, mock transfected or transfected with rat LC3B, and from HT-1080 and A20 cells, untreated or chloroquine-treated 50 µM, overnight, using LC3B Antibody. Entrez-Gene ID # 81631 Swiss-Prot Acc. # Q9GZQ8 Chloroquine is a chemotherapeutic agent for the clinical treatment of malaria. Chloroquine is able to bind to DNA, and inhibit DNA replication and RNA synthesis which in turn results in cell death. The effect of Chloroquine may also be related to the formation of a toxic heme-Chloroquine complex. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 u M, overnight; right) using LC3B Antibody (green) and β-Catenin (L54E2) Mouse m Ab (Alexa Fluor® 555 Conjugate) #5612 (red). Chloroquine cell signalling Chloroquine Eliminates Cancer Stem Cells Through Deregulation., LC3B Antibody - Cell Signaling Technology Plaquenil and lupus rashPlaquenil and atenolol safe togetherGeneric plaquenil price Cell Signaling Technology, Inc. Background Chloroquine CQ is a lysosomotropic agent with an extensive range of biological effects 1. Historically known for its anti-malarial activity, chloroquine is a widely used biological research tool for studying autophagy inhibition. Research studies demon- Chloroquine - Cell Signaling Technology. Chloroquine diphosphate ≥99%HPLC Selleck ATM/ATR activator. Cell Signaling Technology. For over a half-century the anti-malarial drug chloroquine CQ has been used as a therapeutic agent, alone or in combination, to treat autoimmune diseases. However, neither the underlying. Chloroquine Fusion with Lysosome P62-substrate Targeting to autophagosome Targeting for degradation Misfolded proteins Cell death signaling proteins Cell survival signaling proteins Cell survival Cell survival Cell death Cell survival Cell death Cell death Result of complete process Failure Figure 1. Incubate membrane and primary antibody at the appropriate dilution and diluent as recommended in the product datasheet in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C. Wash three times for 5 min each with 15 ml of TBST.